Discovery of a Family of Mixed Lineage Kinase Domain-like Proteins in Plants and Their Role in Innate Immune Signaling.
Identifieur interne : 000178 ( Main/Exploration ); précédent : 000177; suivant : 000179Discovery of a Family of Mixed Lineage Kinase Domain-like Proteins in Plants and Their Role in Innate Immune Signaling.
Auteurs : Lisa K. Mahdi [Allemagne] ; Menghang Huang [République populaire de Chine] ; Xiaoxiao Zhang [République populaire de Chine] ; Ryohei Thomas Nakano [Allemagne] ; Leïla Brulé Kopp [France] ; Isabel M L. Saur [Allemagne] ; Florence Jacob [France] ; Viera Kovacova [Allemagne] ; Dmitry Lapin [Allemagne] ; Jane E. Parker [Allemagne] ; James M. Murphy [Australie] ; Kay Hofmann [Allemagne] ; Paul Schulze-Lefert [Allemagne] ; Jijie Chai [Allemagne] ; Takaki Maekawa [Allemagne]Source :
- Cell host & microbe [ 1934-6069 ] ; 2020.
Abstract
HeLo domain-containing mixed lineage kinase domain-like protein (MLKL), a pseudokinase, mediates necroptotic cell death in animals. Here, we report the discovery of a conserved protein family across seed plants that structurally resembles vertebrate MLKL. The Arabidopsis genome encodes three MLKLs (AtMLKLs) with overlapping functions in disease resistance mediated by Toll-interleukin 1-receptor domain intracellular immune receptors (TNLs). The HeLo domain of AtMLKLs confers cell death activity but is dispensable for immunity. Cryo-EM structures reveal a tetrameric configuration, in which the HeLo domain is buried, suggestive of an auto-repressed complex. The mobility of AtMLKL1 along microtubules is reduced by chitin, a fungal immunity-triggering molecule. An AtMLKL1 phosphomimetic variant exhibiting reduced mobility enhances immunity. Coupled with the predicted presence of HeLo domains in plant helper NLRs, our data reveal the importance of HeLo domain proteins for TNL-dependent immunity and argue for a cell death-independent immune mechanism mediated by MLKLs.
DOI: 10.1016/j.chom.2020.08.012
PubMed: 33053377
Affiliations:
- Allemagne, Australie, France, République populaire de Chine
- District de Cologne, Rhénanie-du-Nord-Westphalie, Victoria (État), Île-de-France
- Cologne, Melbourne, Orsay, Paris, Pékin
- Université Paris-Sud, Université de Melbourne
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Saur</name><affiliation wicri:level="3"><nlm:affiliation>Max Planck Institute for Plant Breeding Research, 50829 Cologne, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Max Planck Institute for Plant Breeding Research, 50829 Cologne</wicri:regionArea><placeName><region type="land" nuts="1">Rhénanie-du-Nord-Westphalie</region><region type="district" nuts="2">District de Cologne</region><settlement type="city">Cologne</settlement></placeName></affiliation></author><author><name sortKey="Jacob, Florence" sort="Jacob, Florence" uniqKey="Jacob F" first="Florence" last="Jacob">Florence Jacob</name><affiliation wicri:level="4"><nlm:affiliation>Max Planck Institute for Plant Breeding Research, 50829 Cologne, Germany; Institute of Plant Sciences Paris-Saclay, Centre National de la Recherche Scientifique, Institut National de la Recherche Agronomique, Université Paris Sud, Université d'Evry, Université Paris Diderot, Sorbonne Paris-Cité, Université Paris-Saclay, 91405 Orsay, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Max Planck Institute for Plant Breeding Research, 50829 Cologne, Germany; Institute of Plant Sciences Paris-Saclay, Centre National de la Recherche Scientifique, Institut National de la Recherche Agronomique, Université Paris Sud, Université d'Evry, Université Paris Diderot, Sorbonne Paris-Cité, Université Paris-Saclay, 91405 Orsay</wicri:regionArea><placeName><region type="region" nuts="2">Île-de-France</region><settlement type="city">Orsay</settlement></placeName><orgName type="university">Université Paris-Sud</orgName></affiliation></author><author><name sortKey="Kovacova, Viera" sort="Kovacova, Viera" uniqKey="Kovacova V" first="Viera" last="Kovacova">Viera Kovacova</name><affiliation wicri:level="3"><nlm:affiliation>University of Cologne, Cologne Excellence Cluster on Cellular Stress Responses in Aging-Associated Diseases (CECAD), University of Cologne, 50931 Cologne, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>University of Cologne, Cologne Excellence Cluster on Cellular Stress Responses in Aging-Associated Diseases (CECAD), University of Cologne, 50931 Cologne</wicri:regionArea><placeName><region type="land" nuts="1">Rhénanie-du-Nord-Westphalie</region><region type="district" nuts="2">District de Cologne</region><settlement type="city">Cologne</settlement></placeName></affiliation></author><author><name sortKey="Lapin, Dmitry" sort="Lapin, Dmitry" uniqKey="Lapin D" first="Dmitry" last="Lapin">Dmitry Lapin</name><affiliation wicri:level="3"><nlm:affiliation>Max Planck Institute for Plant Breeding Research, 50829 Cologne, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Max Planck Institute for Plant Breeding Research, 50829 Cologne</wicri:regionArea><placeName><region type="land" nuts="1">Rhénanie-du-Nord-Westphalie</region><region type="district" nuts="2">District de Cologne</region><settlement type="city">Cologne</settlement></placeName></affiliation></author><author><name sortKey="Parker, Jane E" sort="Parker, Jane E" uniqKey="Parker J" first="Jane E" last="Parker">Jane E. Parker</name><affiliation wicri:level="3"><nlm:affiliation>Max Planck Institute for Plant Breeding Research, 50829 Cologne, Germany; Cluster of Excellence on Plant Sciences (CEPLAS), Max Planck Institute for Plant Breeding Research, 50829 Cologne, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Max Planck Institute for Plant Breeding Research, 50829 Cologne, Germany; Cluster of Excellence on Plant Sciences (CEPLAS), Max Planck Institute for Plant Breeding Research, 50829 Cologne</wicri:regionArea><placeName><region type="land" nuts="1">Rhénanie-du-Nord-Westphalie</region><region type="district" nuts="2">District de Cologne</region><settlement type="city">Cologne</settlement></placeName></affiliation></author><author><name sortKey="Murphy, James M" sort="Murphy, James M" uniqKey="Murphy J" first="James M" last="Murphy">James M. Murphy</name><affiliation wicri:level="4"><nlm:affiliation>Walter and Eliza Hall Institute of Medical Research, 1G Royal Parade, Parkville, VIC 3052, Australia; Department of Medical Biology, University of Melbourne, Parkville, VIC 3052, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Walter and Eliza Hall Institute of Medical Research, 1G Royal Parade, Parkville, VIC 3052, Australia; Department of Medical Biology, University of Melbourne, Parkville, VIC 3052</wicri:regionArea><orgName type="university">Université de Melbourne</orgName><placeName><settlement type="city">Melbourne</settlement><region type="état">Victoria (État)</region></placeName></affiliation></author><author><name sortKey="Hofmann, Kay" sort="Hofmann, Kay" uniqKey="Hofmann K" first="Kay" last="Hofmann">Kay Hofmann</name><affiliation wicri:level="3"><nlm:affiliation>Institute for Genetics, University of Cologne, 50674 Cologne, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institute for Genetics, University of Cologne, 50674 Cologne</wicri:regionArea><placeName><region type="land" nuts="1">Rhénanie-du-Nord-Westphalie</region><region type="district" nuts="2">District de Cologne</region><settlement type="city">Cologne</settlement></placeName></affiliation></author><author><name sortKey="Schulze Lefert, Paul" sort="Schulze Lefert, Paul" uniqKey="Schulze Lefert P" first="Paul" last="Schulze-Lefert">Paul Schulze-Lefert</name><affiliation wicri:level="3"><nlm:affiliation>Max Planck Institute for Plant Breeding Research, 50829 Cologne, Germany; Cluster of Excellence on Plant Sciences (CEPLAS), Max Planck Institute for Plant Breeding Research, 50829 Cologne, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Max Planck Institute for Plant Breeding Research, 50829 Cologne, Germany; Cluster of Excellence on Plant Sciences (CEPLAS), Max Planck Institute for Plant Breeding Research, 50829 Cologne</wicri:regionArea><placeName><region type="land" nuts="1">Rhénanie-du-Nord-Westphalie</region><region type="district" nuts="2">District de Cologne</region><settlement type="city">Cologne</settlement></placeName></affiliation></author><author><name sortKey="Chai, Jijie" sort="Chai, Jijie" uniqKey="Chai J" first="Jijie" last="Chai">Jijie Chai</name><affiliation wicri:level="3"><nlm:affiliation>Max Planck Institute for Plant Breeding Research, 50829 Cologne, Germany; Beijing Advanced Innovation Center for Structural Biology, Tsinghua-Peking Joint Center for Life Sciences, Center for Plant Biology, School of Life Sciences, Tsinghua University, 100084 Beijing, China; Cluster of Excellence on Plant Sciences (CEPLAS), Max Planck Institute for Plant Breeding Research, 50829 Cologne, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Max Planck Institute for Plant Breeding Research, 50829 Cologne, Germany; Beijing Advanced Innovation Center for Structural Biology, Tsinghua-Peking Joint Center for Life Sciences, Center for Plant Biology, School of Life Sciences, Tsinghua University, 100084 Beijing, China; Cluster of Excellence on Plant Sciences (CEPLAS), Max Planck Institute for Plant Breeding Research, 50829 Cologne</wicri:regionArea><placeName><region type="land" nuts="1">Rhénanie-du-Nord-Westphalie</region><region type="district" nuts="2">District de Cologne</region><settlement type="city">Cologne</settlement></placeName></affiliation></author><author><name sortKey="Maekawa, Takaki" sort="Maekawa, Takaki" uniqKey="Maekawa T" first="Takaki" last="Maekawa">Takaki Maekawa</name><affiliation wicri:level="3"><nlm:affiliation>Max Planck Institute for Plant Breeding Research, 50829 Cologne, Germany. Electronic address: maekawa@mpipz.mpg.de.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Max Planck Institute for Plant Breeding Research, 50829 Cologne</wicri:regionArea><placeName><region type="land" nuts="1">Rhénanie-du-Nord-Westphalie</region><region type="district" nuts="2">District de Cologne</region><settlement type="city">Cologne</settlement></placeName></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2020">2020</date><idno type="RBID">pubmed:33053377</idno><idno type="pmid">33053377</idno><idno type="doi">10.1016/j.chom.2020.08.012</idno><idno type="wicri:Area/Main/Corpus">000029</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000029</idno><idno type="wicri:Area/Main/Curation">000029</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000029</idno><idno type="wicri:Area/Main/Exploration">000029</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Discovery of a Family of Mixed Lineage Kinase Domain-like Proteins in Plants and Their Role in Innate Immune Signaling.</title><author><name sortKey="Mahdi, Lisa K" sort="Mahdi, Lisa K" uniqKey="Mahdi L" first="Lisa K" last="Mahdi">Lisa K. Mahdi</name><affiliation wicri:level="3"><nlm:affiliation>Max Planck Institute for Plant Breeding Research, 50829 Cologne, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Max Planck Institute for Plant Breeding Research, 50829 Cologne</wicri:regionArea><placeName><region type="land" nuts="1">Rhénanie-du-Nord-Westphalie</region><region type="district" nuts="2">District de Cologne</region><settlement type="city">Cologne</settlement></placeName></affiliation></author><author><name sortKey="Huang, Menghang" sort="Huang, Menghang" uniqKey="Huang M" first="Menghang" last="Huang">Menghang Huang</name><affiliation wicri:level="1"><nlm:affiliation>Beijing Advanced Innovation Center for Structural Biology, Tsinghua-Peking Joint Center for Life Sciences, Center for Plant Biology, School of Life Sciences, Tsinghua University, 100084 Beijing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Beijing Advanced Innovation Center for Structural Biology, Tsinghua-Peking Joint Center for Life Sciences, Center for Plant Biology, School of Life Sciences, Tsinghua University, 100084 Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Zhang, Xiaoxiao" sort="Zhang, Xiaoxiao" uniqKey="Zhang X" first="Xiaoxiao" last="Zhang">Xiaoxiao Zhang</name><affiliation wicri:level="1"><nlm:affiliation>Beijing Advanced Innovation Center for Structural Biology, Tsinghua-Peking Joint Center for Life Sciences, Center for Plant Biology, School of Life Sciences, Tsinghua University, 100084 Beijing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Beijing Advanced Innovation Center for Structural Biology, Tsinghua-Peking Joint Center for Life Sciences, Center for Plant Biology, School of Life Sciences, Tsinghua University, 100084 Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Nakano, Ryohei Thomas" sort="Nakano, Ryohei Thomas" uniqKey="Nakano R" first="Ryohei Thomas" last="Nakano">Ryohei Thomas Nakano</name><affiliation wicri:level="3"><nlm:affiliation>Max Planck Institute for Plant Breeding Research, 50829 Cologne, Germany; Cluster of Excellence on Plant Sciences (CEPLAS), Max Planck Institute for Plant Breeding Research, 50829 Cologne, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Max Planck Institute for Plant Breeding Research, 50829 Cologne, Germany; Cluster of Excellence on Plant Sciences (CEPLAS), Max Planck Institute for Plant Breeding Research, 50829 Cologne</wicri:regionArea><placeName><region type="land" nuts="1">Rhénanie-du-Nord-Westphalie</region><region type="district" nuts="2">District de Cologne</region><settlement type="city">Cologne</settlement></placeName></affiliation></author><author><name sortKey="Kopp, Leila Brule" sort="Kopp, Leila Brule" uniqKey="Kopp L" first="Leïla Brulé" last="Kopp">Leïla Brulé Kopp</name><affiliation wicri:level="3"><nlm:affiliation>Max Planck Institute for Plant Breeding Research, 50829 Cologne, Germany; École normale supérieure Paris, 75230 Paris Cedex 05, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Max Planck Institute for Plant Breeding Research, 50829 Cologne, Germany; École normale supérieure Paris, 75230 Paris Cedex 05</wicri:regionArea><placeName><region type="region" nuts="2">Île-de-France</region><settlement type="city">Paris</settlement></placeName></affiliation></author><author><name sortKey="Saur, Isabel M L" sort="Saur, Isabel M L" uniqKey="Saur I" first="Isabel M L" last="Saur">Isabel M L. Saur</name><affiliation wicri:level="3"><nlm:affiliation>Max Planck Institute for Plant Breeding Research, 50829 Cologne, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Max Planck Institute for Plant Breeding Research, 50829 Cologne</wicri:regionArea><placeName><region type="land" nuts="1">Rhénanie-du-Nord-Westphalie</region><region type="district" nuts="2">District de Cologne</region><settlement type="city">Cologne</settlement></placeName></affiliation></author><author><name sortKey="Jacob, Florence" sort="Jacob, Florence" uniqKey="Jacob F" first="Florence" last="Jacob">Florence Jacob</name><affiliation wicri:level="4"><nlm:affiliation>Max Planck Institute for Plant Breeding Research, 50829 Cologne, Germany; Institute of Plant Sciences Paris-Saclay, Centre National de la Recherche Scientifique, Institut National de la Recherche Agronomique, Université Paris Sud, Université d'Evry, Université Paris Diderot, Sorbonne Paris-Cité, Université Paris-Saclay, 91405 Orsay, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Max Planck Institute for Plant Breeding Research, 50829 Cologne, Germany; Institute of Plant Sciences Paris-Saclay, Centre National de la Recherche Scientifique, Institut National de la Recherche Agronomique, Université Paris Sud, Université d'Evry, Université Paris Diderot, Sorbonne Paris-Cité, Université Paris-Saclay, 91405 Orsay</wicri:regionArea><placeName><region type="region" nuts="2">Île-de-France</region><settlement type="city">Orsay</settlement></placeName><orgName type="university">Université Paris-Sud</orgName></affiliation></author><author><name sortKey="Kovacova, Viera" sort="Kovacova, Viera" uniqKey="Kovacova V" first="Viera" last="Kovacova">Viera Kovacova</name><affiliation wicri:level="3"><nlm:affiliation>University of Cologne, Cologne Excellence Cluster on Cellular Stress Responses in Aging-Associated Diseases (CECAD), University of Cologne, 50931 Cologne, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>University of Cologne, Cologne Excellence Cluster on Cellular Stress Responses in Aging-Associated Diseases (CECAD), University of Cologne, 50931 Cologne</wicri:regionArea><placeName><region type="land" nuts="1">Rhénanie-du-Nord-Westphalie</region><region type="district" nuts="2">District de Cologne</region><settlement type="city">Cologne</settlement></placeName></affiliation></author><author><name sortKey="Lapin, Dmitry" sort="Lapin, Dmitry" uniqKey="Lapin D" first="Dmitry" last="Lapin">Dmitry Lapin</name><affiliation wicri:level="3"><nlm:affiliation>Max Planck Institute for Plant Breeding Research, 50829 Cologne, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Max Planck Institute for Plant Breeding Research, 50829 Cologne</wicri:regionArea><placeName><region type="land" nuts="1">Rhénanie-du-Nord-Westphalie</region><region type="district" nuts="2">District de Cologne</region><settlement type="city">Cologne</settlement></placeName></affiliation></author><author><name sortKey="Parker, Jane E" sort="Parker, Jane E" uniqKey="Parker J" first="Jane E" last="Parker">Jane E. Parker</name><affiliation wicri:level="3"><nlm:affiliation>Max Planck Institute for Plant Breeding Research, 50829 Cologne, Germany; Cluster of Excellence on Plant Sciences (CEPLAS), Max Planck Institute for Plant Breeding Research, 50829 Cologne, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Max Planck Institute for Plant Breeding Research, 50829 Cologne, Germany; Cluster of Excellence on Plant Sciences (CEPLAS), Max Planck Institute for Plant Breeding Research, 50829 Cologne</wicri:regionArea><placeName><region type="land" nuts="1">Rhénanie-du-Nord-Westphalie</region><region type="district" nuts="2">District de Cologne</region><settlement type="city">Cologne</settlement></placeName></affiliation></author><author><name sortKey="Murphy, James M" sort="Murphy, James M" uniqKey="Murphy J" first="James M" last="Murphy">James M. Murphy</name><affiliation wicri:level="4"><nlm:affiliation>Walter and Eliza Hall Institute of Medical Research, 1G Royal Parade, Parkville, VIC 3052, Australia; Department of Medical Biology, University of Melbourne, Parkville, VIC 3052, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Walter and Eliza Hall Institute of Medical Research, 1G Royal Parade, Parkville, VIC 3052, Australia; Department of Medical Biology, University of Melbourne, Parkville, VIC 3052</wicri:regionArea><orgName type="university">Université de Melbourne</orgName><placeName><settlement type="city">Melbourne</settlement><region type="état">Victoria (État)</region></placeName></affiliation></author><author><name sortKey="Hofmann, Kay" sort="Hofmann, Kay" uniqKey="Hofmann K" first="Kay" last="Hofmann">Kay Hofmann</name><affiliation wicri:level="3"><nlm:affiliation>Institute for Genetics, University of Cologne, 50674 Cologne, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institute for Genetics, University of Cologne, 50674 Cologne</wicri:regionArea><placeName><region type="land" nuts="1">Rhénanie-du-Nord-Westphalie</region><region type="district" nuts="2">District de Cologne</region><settlement type="city">Cologne</settlement></placeName></affiliation></author><author><name sortKey="Schulze Lefert, Paul" sort="Schulze Lefert, Paul" uniqKey="Schulze Lefert P" first="Paul" last="Schulze-Lefert">Paul Schulze-Lefert</name><affiliation wicri:level="3"><nlm:affiliation>Max Planck Institute for Plant Breeding Research, 50829 Cologne, Germany; Cluster of Excellence on Plant Sciences (CEPLAS), Max Planck Institute for Plant Breeding Research, 50829 Cologne, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Max Planck Institute for Plant Breeding Research, 50829 Cologne, Germany; Cluster of Excellence on Plant Sciences (CEPLAS), Max Planck Institute for Plant Breeding Research, 50829 Cologne</wicri:regionArea><placeName><region type="land" nuts="1">Rhénanie-du-Nord-Westphalie</region><region type="district" nuts="2">District de Cologne</region><settlement type="city">Cologne</settlement></placeName></affiliation></author><author><name sortKey="Chai, Jijie" sort="Chai, Jijie" uniqKey="Chai J" first="Jijie" last="Chai">Jijie Chai</name><affiliation wicri:level="3"><nlm:affiliation>Max Planck Institute for Plant Breeding Research, 50829 Cologne, Germany; Beijing Advanced Innovation Center for Structural Biology, Tsinghua-Peking Joint Center for Life Sciences, Center for Plant Biology, School of Life Sciences, Tsinghua University, 100084 Beijing, China; Cluster of Excellence on Plant Sciences (CEPLAS), Max Planck Institute for Plant Breeding Research, 50829 Cologne, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Max Planck Institute for Plant Breeding Research, 50829 Cologne, Germany; Beijing Advanced Innovation Center for Structural Biology, Tsinghua-Peking Joint Center for Life Sciences, Center for Plant Biology, School of Life Sciences, Tsinghua University, 100084 Beijing, China; Cluster of Excellence on Plant Sciences (CEPLAS), Max Planck Institute for Plant Breeding Research, 50829 Cologne</wicri:regionArea><placeName><region type="land" nuts="1">Rhénanie-du-Nord-Westphalie</region><region type="district" nuts="2">District de Cologne</region><settlement type="city">Cologne</settlement></placeName></affiliation></author><author><name sortKey="Maekawa, Takaki" sort="Maekawa, Takaki" uniqKey="Maekawa T" first="Takaki" last="Maekawa">Takaki Maekawa</name><affiliation wicri:level="3"><nlm:affiliation>Max Planck Institute for Plant Breeding Research, 50829 Cologne, Germany. Electronic address: maekawa@mpipz.mpg.de.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Max Planck Institute for Plant Breeding Research, 50829 Cologne</wicri:regionArea><placeName><region type="land" nuts="1">Rhénanie-du-Nord-Westphalie</region><region type="district" nuts="2">District de Cologne</region><settlement type="city">Cologne</settlement></placeName></affiliation></author></analytic><series><title level="j">Cell host & microbe</title><idno type="eISSN">1934-6069</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">HeLo domain-containing mixed lineage kinase domain-like protein (MLKL), a pseudokinase, mediates necroptotic cell death in animals. Here, we report the discovery of a conserved protein family across seed plants that structurally resembles vertebrate MLKL. The Arabidopsis genome encodes three MLKLs (AtMLKLs) with overlapping functions in disease resistance mediated by Toll-interleukin 1-receptor domain intracellular immune receptors (TNLs). The HeLo domain of AtMLKLs confers cell death activity but is dispensable for immunity. Cryo-EM structures reveal a tetrameric configuration, in which the HeLo domain is buried, suggestive of an auto-repressed complex. The mobility of AtMLKL1 along microtubules is reduced by chitin, a fungal immunity-triggering molecule. An AtMLKL1 phosphomimetic variant exhibiting reduced mobility enhances immunity. Coupled with the predicted presence of HeLo domains in plant helper NLRs, our data reveal the importance of HeLo domain proteins for TNL-dependent immunity and argue for a cell death-independent immune mechanism mediated by MLKLs.</div></front></TEI><pubmed><MedlineCitation Status="Publisher" Owner="NLM"><PMID Version="1">33053377</PMID><DateRevised><Year>2020</Year><Month>10</Month><Day>14</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1934-6069</ISSN><JournalIssue CitedMedium="Internet"><PubDate><Year>2020</Year><Month>Sep</Month><Day>30</Day></PubDate></JournalIssue><Title>Cell host & microbe</Title><ISOAbbreviation>Cell Host Microbe</ISOAbbreviation></Journal><ArticleTitle>Discovery of a Family of Mixed Lineage Kinase Domain-like Proteins in Plants and Their Role in Innate Immune Signaling.</ArticleTitle><ELocationID EIdType="pii" ValidYN="Y">S1931-3128(20)30467-4</ELocationID><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.chom.2020.08.012</ELocationID><Abstract><AbstractText>HeLo domain-containing mixed lineage kinase domain-like protein (MLKL), a pseudokinase, mediates necroptotic cell death in animals. Here, we report the discovery of a conserved protein family across seed plants that structurally resembles vertebrate MLKL. The Arabidopsis genome encodes three MLKLs (AtMLKLs) with overlapping functions in disease resistance mediated by Toll-interleukin 1-receptor domain intracellular immune receptors (TNLs). The HeLo domain of AtMLKLs confers cell death activity but is dispensable for immunity. Cryo-EM structures reveal a tetrameric configuration, in which the HeLo domain is buried, suggestive of an auto-repressed complex. The mobility of AtMLKL1 along microtubules is reduced by chitin, a fungal immunity-triggering molecule. An AtMLKL1 phosphomimetic variant exhibiting reduced mobility enhances immunity. Coupled with the predicted presence of HeLo domains in plant helper NLRs, our data reveal the importance of HeLo domain proteins for TNL-dependent immunity and argue for a cell death-independent immune mechanism mediated by MLKLs.</AbstractText><CopyrightInformation>Copyright © 2020 Elsevier Inc. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Mahdi</LastName><ForeName>Lisa K</ForeName><Initials>LK</Initials><AffiliationInfo><Affiliation>Max Planck Institute for Plant Breeding Research, 50829 Cologne, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Huang</LastName><ForeName>Menghang</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Beijing Advanced Innovation Center for Structural Biology, Tsinghua-Peking Joint Center for Life Sciences, Center for Plant Biology, School of Life Sciences, Tsinghua University, 100084 Beijing, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhang</LastName><ForeName>Xiaoxiao</ForeName><Initials>X</Initials><AffiliationInfo><Affiliation>Beijing Advanced Innovation Center for Structural Biology, Tsinghua-Peking Joint Center for Life Sciences, Center for Plant Biology, School of Life Sciences, Tsinghua University, 100084 Beijing, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Nakano</LastName><ForeName>Ryohei Thomas</ForeName><Initials>RT</Initials><AffiliationInfo><Affiliation>Max Planck Institute for Plant Breeding Research, 50829 Cologne, Germany; Cluster of Excellence on Plant Sciences (CEPLAS), Max Planck Institute for Plant Breeding Research, 50829 Cologne, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kopp</LastName><ForeName>Leïla Brulé</ForeName><Initials>LB</Initials><AffiliationInfo><Affiliation>Max Planck Institute for Plant Breeding Research, 50829 Cologne, Germany; École normale supérieure Paris, 75230 Paris Cedex 05, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Saur</LastName><ForeName>Isabel M L</ForeName><Initials>IML</Initials><AffiliationInfo><Affiliation>Max Planck Institute for Plant Breeding Research, 50829 Cologne, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Jacob</LastName><ForeName>Florence</ForeName><Initials>F</Initials><AffiliationInfo><Affiliation>Max Planck Institute for Plant Breeding Research, 50829 Cologne, Germany; Institute of Plant Sciences Paris-Saclay, Centre National de la Recherche Scientifique, Institut National de la Recherche Agronomique, Université Paris Sud, Université d'Evry, Université Paris Diderot, Sorbonne Paris-Cité, Université Paris-Saclay, 91405 Orsay, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kovacova</LastName><ForeName>Viera</ForeName><Initials>V</Initials><AffiliationInfo><Affiliation>University of Cologne, Cologne Excellence Cluster on Cellular Stress Responses in Aging-Associated Diseases (CECAD), University of Cologne, 50931 Cologne, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lapin</LastName><ForeName>Dmitry</ForeName><Initials>D</Initials><AffiliationInfo><Affiliation>Max Planck Institute for Plant Breeding Research, 50829 Cologne, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Parker</LastName><ForeName>Jane E</ForeName><Initials>JE</Initials><AffiliationInfo><Affiliation>Max Planck Institute for Plant Breeding Research, 50829 Cologne, Germany; Cluster of Excellence on Plant Sciences (CEPLAS), Max Planck Institute for Plant Breeding Research, 50829 Cologne, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Murphy</LastName><ForeName>James M</ForeName><Initials>JM</Initials><AffiliationInfo><Affiliation>Walter and Eliza Hall Institute of Medical Research, 1G Royal Parade, Parkville, VIC 3052, Australia; Department of Medical Biology, University of Melbourne, Parkville, VIC 3052, Australia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Hofmann</LastName><ForeName>Kay</ForeName><Initials>K</Initials><AffiliationInfo><Affiliation>Institute for Genetics, University of Cologne, 50674 Cologne, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Schulze-Lefert</LastName><ForeName>Paul</ForeName><Initials>P</Initials><AffiliationInfo><Affiliation>Max Planck Institute for Plant Breeding Research, 50829 Cologne, Germany; Cluster of Excellence on Plant Sciences (CEPLAS), Max Planck Institute for Plant Breeding Research, 50829 Cologne, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Chai</LastName><ForeName>Jijie</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Max Planck Institute for Plant Breeding Research, 50829 Cologne, Germany; Beijing Advanced Innovation Center for Structural Biology, Tsinghua-Peking Joint Center for Life Sciences, Center for Plant Biology, School of Life Sciences, Tsinghua University, 100084 Beijing, China; Cluster of Excellence on Plant Sciences (CEPLAS), Max Planck Institute for Plant Breeding Research, 50829 Cologne, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Maekawa</LastName><ForeName>Takaki</ForeName><Initials>T</Initials><AffiliationInfo><Affiliation>Max Planck Institute for Plant Breeding Research, 50829 Cologne, Germany. Electronic address: maekawa@mpipz.mpg.de.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>09</Month><Day>30</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Cell Host Microbe</MedlineTA><NlmUniqueID>101302316</NlmUniqueID><ISSNLinking>1931-3128</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Cell death</Keyword><Keyword MajorTopicYN="N">HeLo domain</Keyword><Keyword MajorTopicYN="N">MLKL</Keyword><Keyword MajorTopicYN="N">cryo-electron microscopy structures</Keyword><Keyword MajorTopicYN="N">microtubules</Keyword><Keyword MajorTopicYN="N">necroptosis</Keyword><Keyword MajorTopicYN="N">toll-interleukin1-receptor domain intracellular immune receptors</Keyword></KeywordList><CoiStatement>Declaration of Interests The authors declare no competing interests.</CoiStatement></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2020</Year><Month>03</Month><Day>29</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2020</Year><Month>06</Month><Day>05</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2020</Year><Month>08</Month><Day>14</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2020</Year><Month>10</Month><Day>14</Day><Hour>20</Hour><Minute>5</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2020</Year><Month>10</Month><Day>15</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>10</Month><Day>15</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>aheadofprint</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">33053377</ArticleId><ArticleId IdType="pii">S1931-3128(20)30467-4</ArticleId><ArticleId IdType="doi">10.1016/j.chom.2020.08.012</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Allemagne</li><li>Australie</li><li>France</li><li>République populaire de Chine</li></country><region><li>District de Cologne</li><li>Rhénanie-du-Nord-Westphalie</li><li>Victoria (État)</li><li>Île-de-France</li></region><settlement><li>Cologne</li><li>Melbourne</li><li>Orsay</li><li>Paris</li><li>Pékin</li></settlement><orgName><li>Université Paris-Sud</li><li>Université de Melbourne</li></orgName></list><tree><country name="Allemagne"><region name="Rhénanie-du-Nord-Westphalie"><name sortKey="Mahdi, Lisa K" sort="Mahdi, Lisa K" uniqKey="Mahdi L" first="Lisa K" last="Mahdi">Lisa K. Mahdi</name></region><name sortKey="Chai, Jijie" sort="Chai, Jijie" uniqKey="Chai J" first="Jijie" last="Chai">Jijie Chai</name><name sortKey="Hofmann, Kay" sort="Hofmann, Kay" uniqKey="Hofmann K" first="Kay" last="Hofmann">Kay Hofmann</name><name sortKey="Kovacova, Viera" sort="Kovacova, Viera" uniqKey="Kovacova V" first="Viera" last="Kovacova">Viera Kovacova</name><name sortKey="Lapin, Dmitry" sort="Lapin, Dmitry" uniqKey="Lapin D" first="Dmitry" last="Lapin">Dmitry Lapin</name><name sortKey="Maekawa, Takaki" sort="Maekawa, Takaki" uniqKey="Maekawa T" first="Takaki" last="Maekawa">Takaki Maekawa</name><name sortKey="Nakano, Ryohei Thomas" sort="Nakano, Ryohei Thomas" uniqKey="Nakano R" first="Ryohei Thomas" last="Nakano">Ryohei Thomas Nakano</name><name sortKey="Parker, Jane E" sort="Parker, Jane E" uniqKey="Parker J" first="Jane E" last="Parker">Jane E. Parker</name><name sortKey="Saur, Isabel M L" sort="Saur, Isabel M L" uniqKey="Saur I" first="Isabel M L" last="Saur">Isabel M L. Saur</name><name sortKey="Schulze Lefert, Paul" sort="Schulze Lefert, Paul" uniqKey="Schulze Lefert P" first="Paul" last="Schulze-Lefert">Paul Schulze-Lefert</name></country><country name="République populaire de Chine"><noRegion><name sortKey="Huang, Menghang" sort="Huang, Menghang" uniqKey="Huang M" first="Menghang" last="Huang">Menghang Huang</name></noRegion><name sortKey="Zhang, Xiaoxiao" sort="Zhang, Xiaoxiao" uniqKey="Zhang X" first="Xiaoxiao" last="Zhang">Xiaoxiao Zhang</name></country><country name="France"><region name="Île-de-France"><name sortKey="Kopp, Leila Brule" sort="Kopp, Leila Brule" uniqKey="Kopp L" first="Leïla Brulé" last="Kopp">Leïla Brulé Kopp</name></region><name sortKey="Jacob, Florence" sort="Jacob, Florence" uniqKey="Jacob F" first="Florence" last="Jacob">Florence Jacob</name></country><country name="Australie"><region name="Victoria (État)"><name sortKey="Murphy, James M" sort="Murphy, James M" uniqKey="Murphy J" first="James M" last="Murphy">James M. 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